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mko2 gene  (Addgene inc)


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    Structured Review

    Addgene inc mko2 gene
    Characteristics of fluorescent proteins used in this study.
    Mko2 Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mko2 gene/product/Addgene inc
    Average 91 stars, based on 2 article reviews
    mko2 gene - by Bioz Stars, 2026-03
    91/100 stars

    Images

    1) Product Images from "Markerless bacterial artificial chromosome manipulation method by red proteins of phage λ mediated homologous recombination utilizing fluorescent proteins for both positive and counter selection"

    Article Title: Markerless bacterial artificial chromosome manipulation method by red proteins of phage λ mediated homologous recombination utilizing fluorescent proteins for both positive and counter selection

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2023.e18983

    Characteristics of fluorescent proteins used in this study.
    Figure Legend Snippet: Characteristics of fluorescent proteins used in this study.

    Techniques Used:

    Optimization of template plasmids. pAmp-9mScarlet without the terminator rrnB T1 was constructed (A). mScarlet protein expression in the pelleted HTS16CR cells was observed on an LED transilluminator after the cells were transformed with pAmp-9mScarlet (+) or pAmp-9mScarlet without a terminator (−) following overnight cultivation in 2 mL of LB medium at 37 °C (B) . Additionally, the plasmid yields of 2 mL of HST16CR cultures transformed with pAmp-9mScarlet (+) (with terminator) and pAmp-9mScarlet (−) (without terminator) were compared (C). The fluorescent protein gene (FP), EGFP, mClover3, YPet, mOrange, mKO2, or mScarlet were ligated into a plasmid (D), which has the same backbone as pAmp-9mScarlet, except for the fluorescent gene, as shown in . E. coli , HST16CR, or HST08 were transformed with these plasmids or pAmp-S as the negative control (control) and then cultivated on LB agar plates at either 30 °C or 37 °C overnight under ampicillin selective pressure (E) (Supplementary fig. 3E-1, -2 and -3). Note that HST08 cells transformed with mKO2 did not grow on the plates either at 30 °C or 37 °C. An unpaired t -test was used to determine the statistical significance. The calculated p values are shown above the compared groups.
    Figure Legend Snippet: Optimization of template plasmids. pAmp-9mScarlet without the terminator rrnB T1 was constructed (A). mScarlet protein expression in the pelleted HTS16CR cells was observed on an LED transilluminator after the cells were transformed with pAmp-9mScarlet (+) or pAmp-9mScarlet without a terminator (−) following overnight cultivation in 2 mL of LB medium at 37 °C (B) . Additionally, the plasmid yields of 2 mL of HST16CR cultures transformed with pAmp-9mScarlet (+) (with terminator) and pAmp-9mScarlet (−) (without terminator) were compared (C). The fluorescent protein gene (FP), EGFP, mClover3, YPet, mOrange, mKO2, or mScarlet were ligated into a plasmid (D), which has the same backbone as pAmp-9mScarlet, except for the fluorescent gene, as shown in . E. coli , HST16CR, or HST08 were transformed with these plasmids or pAmp-S as the negative control (control) and then cultivated on LB agar plates at either 30 °C or 37 °C overnight under ampicillin selective pressure (E) (Supplementary fig. 3E-1, -2 and -3). Note that HST08 cells transformed with mKO2 did not grow on the plates either at 30 °C or 37 °C. An unpaired t -test was used to determine the statistical significance. The calculated p values are shown above the compared groups.

    Techniques Used: Construct, Expressing, Transformation Assay, Plasmid Preparation, Negative Control, Control



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    Image Search Results


    Characteristics of fluorescent proteins used in this study.

    Journal: Heliyon

    Article Title: Markerless bacterial artificial chromosome manipulation method by red proteins of phage λ mediated homologous recombination utilizing fluorescent proteins for both positive and counter selection

    doi: 10.1016/j.heliyon.2023.e18983

    Figure Lengend Snippet: Characteristics of fluorescent proteins used in this study.

    Article Snippet: The mKO2 gene was obtained from mKO2-pBAD gifted by Michael Davidson and Atsushi Miyawaki (#54555, Addgene) [ ].

    Techniques:

    Optimization of template plasmids. pAmp-9mScarlet without the terminator rrnB T1 was constructed (A). mScarlet protein expression in the pelleted HTS16CR cells was observed on an LED transilluminator after the cells were transformed with pAmp-9mScarlet (+) or pAmp-9mScarlet without a terminator (−) following overnight cultivation in 2 mL of LB medium at 37 °C (B) . Additionally, the plasmid yields of 2 mL of HST16CR cultures transformed with pAmp-9mScarlet (+) (with terminator) and pAmp-9mScarlet (−) (without terminator) were compared (C). The fluorescent protein gene (FP), EGFP, mClover3, YPet, mOrange, mKO2, or mScarlet were ligated into a plasmid (D), which has the same backbone as pAmp-9mScarlet, except for the fluorescent gene, as shown in . E. coli , HST16CR, or HST08 were transformed with these plasmids or pAmp-S as the negative control (control) and then cultivated on LB agar plates at either 30 °C or 37 °C overnight under ampicillin selective pressure (E) (Supplementary fig. 3E-1, -2 and -3). Note that HST08 cells transformed with mKO2 did not grow on the plates either at 30 °C or 37 °C. An unpaired t -test was used to determine the statistical significance. The calculated p values are shown above the compared groups.

    Journal: Heliyon

    Article Title: Markerless bacterial artificial chromosome manipulation method by red proteins of phage λ mediated homologous recombination utilizing fluorescent proteins for both positive and counter selection

    doi: 10.1016/j.heliyon.2023.e18983

    Figure Lengend Snippet: Optimization of template plasmids. pAmp-9mScarlet without the terminator rrnB T1 was constructed (A). mScarlet protein expression in the pelleted HTS16CR cells was observed on an LED transilluminator after the cells were transformed with pAmp-9mScarlet (+) or pAmp-9mScarlet without a terminator (−) following overnight cultivation in 2 mL of LB medium at 37 °C (B) . Additionally, the plasmid yields of 2 mL of HST16CR cultures transformed with pAmp-9mScarlet (+) (with terminator) and pAmp-9mScarlet (−) (without terminator) were compared (C). The fluorescent protein gene (FP), EGFP, mClover3, YPet, mOrange, mKO2, or mScarlet were ligated into a plasmid (D), which has the same backbone as pAmp-9mScarlet, except for the fluorescent gene, as shown in . E. coli , HST16CR, or HST08 were transformed with these plasmids or pAmp-S as the negative control (control) and then cultivated on LB agar plates at either 30 °C or 37 °C overnight under ampicillin selective pressure (E) (Supplementary fig. 3E-1, -2 and -3). Note that HST08 cells transformed with mKO2 did not grow on the plates either at 30 °C or 37 °C. An unpaired t -test was used to determine the statistical significance. The calculated p values are shown above the compared groups.

    Article Snippet: The mKO2 gene was obtained from mKO2-pBAD gifted by Michael Davidson and Atsushi Miyawaki (#54555, Addgene) [ ].

    Techniques: Construct, Expressing, Transformation Assay, Plasmid Preparation, Negative Control, Control

    Journal: eLife

    Article Title: Cdc48-like protein of actinobacteria (Cpa) is a novel proteasome interactor in mycobacteria and related organisms

    doi: 10.7554/eLife.34055

    Figure Lengend Snippet:

    Article Snippet: Gene , mKO2 , Thermo Fisher Scientific , NCBI accession: AB359188 , GeneArt synthetic construct.

    Techniques: Construct, Produced, Recombinant, Plasmid Preparation, Expressing, Mutagenesis, Variant Assay, Software